Composite

Part:BBa_K180017:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-21)


Rock-paper-scissors - Scissors primary plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 887
    Illegal NheI site found at 910
    Illegal PstI site found at 2754
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2754
    Illegal NgoMIV site found at 534
    Illegal NgoMIV site found at 3000
    Illegal AgeI site found at 337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2943


Design Notes

BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.


Source

See relevant biobrick component parts (BBa_K180018 and BBa_K180019).

References